Sensitivity and Usefulness of VE1 Immunohistochemical Staining in Acral Melanomas with BRAF Mutation

A validation study for the use VE1 immunohistochemical staining in screening for BRAF mutation in cutaneous malignant melanoma

BRAF V600E mutation analysis has been an important companion diagnostic assay to evaluate prognosis of malignant melanoma and to guide target therapy with BRAF inhibitor. The aim of this study was to assess the sensitivity and specificity of Immunohistochemistry (IHC) using VE1 mutation specific antibody on screening for BRAF V600E mutation patients with cutaneous malignant melanoma. We detecte...

White paper Evaluation of BRAF (V600E) Mutation by Immunohistochemical Staining with anti-BRAF V600E (VE1) Antibody: A Comparison with Sanger Sequencing

White paper Evaluation of BRAF (V600E) Mutation by Immunohistochemical Staining with anti-BRAF V600E (VE1) Antibody: A Comparison with Sanger Sequencing

Frequency of BRAF Mutation and Clinical Relevance for Primary Melanomas

BACKGROUND This study was conducted to clarify the frequency of the BRAF mutation in primary melanomas and its correlation with clinicopathologic parameters. METHODS We analyzed the frequency of BRAF mutation in patients with primary cutaneous melanoma (n=58) or non-cutaneous one (n=27) by performing dual priming oligonucleotide-based multiplex real-time polymerase chain reaction to isolate a...

Assessment of BRAF V600E Status in Colorectal Carcinoma: Tissue-Specific Discordances between Immunohistochemistry and Sequencing.

Although sequencing provides the gold standard for identifying colorectal carcinoma with BRAF V600E mutation, immunohistochemistry (IHC) with the recently developed mouse monoclonal antibody VE1 for BRAF V600E protein has shown promise as a more widely available and rapid method. However, we identified anecdotal discordance between VE1 IHC and sequencing results and therefore analyzed VE1 stain...